amaxa 4d nucleofector program fi 115 Search Results


nucleofector 4d program fi 115  (Amaxa)
86
Amaxa nucleofector 4d program fi 115
Nucleofector 4d Program Fi 115, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+4d+nucleofector+program+fi+115/electroporation/pmc12409989-335-8-7
Average 86 stars, based on 1 article reviews
nucleofector 4d program fi 115 - by Bioz Stars, 2026-09
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amaxa 4d nucleofector  (Amaxa)
86
Amaxa amaxa 4d nucleofector
Amaxa 4d Nucleofector, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+4d+nucleofector+program+fi+115/4d+amaxa+nucleofector/pmc12488502-279-34-34
Average 86 stars, based on 1 article reviews
amaxa 4d nucleofector - by Bioz Stars, 2026-09
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4d nucleofection system  (Amaxa)
86
Amaxa 4d nucleofection system
4d Nucleofection System, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+4d+nucleofector+program+fi+115/4d+amaxa+nucleofection+system/10__1038_slash_s41596___018___0069___7-384-5-4
Average 86 stars, based on 1 article reviews
4d nucleofection system - by Bioz Stars, 2026-09
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unstimulated human t cell kit  (Amaxa)
86
Amaxa unstimulated human t cell kit
Unstimulated Human T Cell Kit, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+4d+nucleofector+program+fi+115/cell+human+kit+t+unstimulated/pmc12409989-335-14-7
Average 86 stars, based on 1 article reviews
unstimulated human t cell kit - by Bioz Stars, 2026-09
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nucleofection  (Amaxa)
86
Amaxa nucleofection
Nucleofection, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+4d+nucleofector+program+fi+115/nucleofection/lam_kentson__2014__characterization_of_runx1_function_in_hematopoiesis-548-7-8
Average 86 stars, based on 1 article reviews
nucleofection - by Bioz Stars, 2026-09
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ficoll-hypaque gradients  (Pharmacia LKB Biotechnology Inc)
90
Pharmacia LKB Biotechnology Inc ficoll-hypaque gradients
Ficoll Hypaque Gradients, supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+4d+nucleofector+program+fi+115/ficoll+hypaque/pmc05239498-139-11-13
Average 90 stars, based on 1 article reviews
ficoll-hypaque gradients - by Bioz Stars, 2026-09
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ampk plasmid  (Addgene inc)
91
Addgene inc ampk plasmid
Ampk Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+4d+nucleofector+program+fi+115/AMPK-%CE%B31-HA+(Plasmid+%2340605)/us08383656-544-9-11
Average 91 stars, based on 1 article reviews
ampk plasmid - by Bioz Stars, 2026-09
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bamhi  (New England Biolabs)
99
New England Biolabs bamhi
Bamhi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+4d+nucleofector+program+fi+115/BamHI/custom%40r0136%4036996244
Average 99 stars, based on 1 article reviews
bamhi - by Bioz Stars, 2026-09
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noti  (New England Biolabs)
99
New England Biolabs noti
Noti, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+4d+nucleofector+program+fi+115/NotI/custom%40r0189%4036996244
Average 99 stars, based on 1 article reviews
noti - by Bioz Stars, 2026-09
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recombinant alt r streptococcus pyogenes cas9 protein v3  (Integrated DNA Technologies)
99
Integrated DNA Technologies recombinant alt r streptococcus pyogenes cas9 protein v3
Protocol Outline for a GMP-Compatible Approach to Induce Tacrolimus Resistance in a CMV-Specific TCP Using Vector-Free <t>CRISPR-Cas9</t> Technology (A) Individual steps of the production process. (B) Representative purities of CMV-specific IFNγ-producing T cells pre - and post-enrichment from PBMCs. (C) Calculated KO efficiency of FKBP12 in CMV-specific TCPs. (D) Expansion rates of electroporated CMV-specific TCPs normalized to expansion rates of the unmodified control from day 7 to day 21 (following electroporation). (E) Different immunosuppressants used for functional analyses and their affected adaptor proteins or receptors and pathways. Classic triple IS consists of a CNI, prednisolone, and MPA.
Recombinant Alt R Streptococcus Pyogenes Cas9 Protein V3, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+4d+nucleofector+program+fi+115/Cas9+Nuclease/pmc07791012-225-36-43
Average 99 stars, based on 1 article reviews
recombinant alt r streptococcus pyogenes cas9 protein v3 - by Bioz Stars, 2026-09
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bglii  (New England Biolabs)
98
New England Biolabs bglii
Protocol Outline for a GMP-Compatible Approach to Induce Tacrolimus Resistance in a CMV-Specific TCP Using Vector-Free <t>CRISPR-Cas9</t> Technology (A) Individual steps of the production process. (B) Representative purities of CMV-specific IFNγ-producing T cells pre - and post-enrichment from PBMCs. (C) Calculated KO efficiency of FKBP12 in CMV-specific TCPs. (D) Expansion rates of electroporated CMV-specific TCPs normalized to expansion rates of the unmodified control from day 7 to day 21 (following electroporation). (E) Different immunosuppressants used for functional analyses and their affected adaptor proteins or receptors and pathways. Classic triple IS consists of a CNI, prednisolone, and MPA.
Bglii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+4d+nucleofector+program+fi+115/BglII/custom%40r0144%4010%2E1101%2F477737
Average 98 stars, based on 1 article reviews
bglii - by Bioz Stars, 2026-09
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xbai  (New England Biolabs)
99
New England Biolabs xbai
Protocol Outline for a GMP-Compatible Approach to Induce Tacrolimus Resistance in a CMV-Specific TCP Using Vector-Free <t>CRISPR-Cas9</t> Technology (A) Individual steps of the production process. (B) Representative purities of CMV-specific IFNγ-producing T cells pre - and post-enrichment from PBMCs. (C) Calculated KO efficiency of FKBP12 in CMV-specific TCPs. (D) Expansion rates of electroporated CMV-specific TCPs normalized to expansion rates of the unmodified control from day 7 to day 21 (following electroporation). (E) Different immunosuppressants used for functional analyses and their affected adaptor proteins or receptors and pathways. Classic triple IS consists of a CNI, prednisolone, and MPA.
Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+4d+nucleofector+program+fi+115/XbaI/custom%40r0145%4010%2E1101%2F477737
Average 99 stars, based on 1 article reviews
xbai - by Bioz Stars, 2026-09
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Protocol Outline for a GMP-Compatible Approach to Induce Tacrolimus Resistance in a CMV-Specific TCP Using Vector-Free CRISPR-Cas9 Technology (A) Individual steps of the production process. (B) Representative purities of CMV-specific IFNγ-producing T cells pre - and post-enrichment from PBMCs. (C) Calculated KO efficiency of FKBP12 in CMV-specific TCPs. (D) Expansion rates of electroporated CMV-specific TCPs normalized to expansion rates of the unmodified control from day 7 to day 21 (following electroporation). (E) Different immunosuppressants used for functional analyses and their affected adaptor proteins or receptors and pathways. Classic triple IS consists of a CNI, prednisolone, and MPA.

Journal: Molecular Therapy

Article Title: CRISPR-Cas9-Edited Tacrolimus-Resistant Antiviral T Cells for Advanced Adoptive Immunotherapy in Transplant Recipients

doi: 10.1016/j.ymthe.2020.09.011

Figure Lengend Snippet: Protocol Outline for a GMP-Compatible Approach to Induce Tacrolimus Resistance in a CMV-Specific TCP Using Vector-Free CRISPR-Cas9 Technology (A) Individual steps of the production process. (B) Representative purities of CMV-specific IFNγ-producing T cells pre - and post-enrichment from PBMCs. (C) Calculated KO efficiency of FKBP12 in CMV-specific TCPs. (D) Expansion rates of electroporated CMV-specific TCPs normalized to expansion rates of the unmodified control from day 7 to day 21 (following electroporation). (E) Different immunosuppressants used for functional analyses and their affected adaptor proteins or receptors and pathways. Classic triple IS consists of a CNI, prednisolone, and MPA.

Article Snippet: 2.5 million antiviral T cells were electroporated on day 7 after isolation using the Amaxa P3 Primary Cell 4D-Nucleofector X Kit L and the Amaxa-Nucleofector-4D (Lonza, program CO-115) to transfer RNP complexes of 30 μg of recombinant Alt-R Streptococcus pyogenes Cas9 protein V3 (Integrated DNA Technologies) precomplexed with 30 μg of in-vitro -transcribed or 15 synt sgRNA (Synthego).

Techniques: Plasmid Preparation, CRISPR, Electroporation, Functional Assay

Cas9 Protein Is Undetectable in FKBP12 KO CMV TCPs Shown are proportions of edited T cells stained for Cas9 protein at different time points after electroporation.

Journal: Molecular Therapy

Article Title: CRISPR-Cas9-Edited Tacrolimus-Resistant Antiviral T Cells for Advanced Adoptive Immunotherapy in Transplant Recipients

doi: 10.1016/j.ymthe.2020.09.011

Figure Lengend Snippet: Cas9 Protein Is Undetectable in FKBP12 KO CMV TCPs Shown are proportions of edited T cells stained for Cas9 protein at different time points after electroporation.

Article Snippet: 2.5 million antiviral T cells were electroporated on day 7 after isolation using the Amaxa P3 Primary Cell 4D-Nucleofector X Kit L and the Amaxa-Nucleofector-4D (Lonza, program CO-115) to transfer RNP complexes of 30 μg of recombinant Alt-R Streptococcus pyogenes Cas9 protein V3 (Integrated DNA Technologies) precomplexed with 30 μg of in-vitro -transcribed or 15 synt sgRNA (Synthego).

Techniques: Staining, Electroporation